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miR-324-5p overexpression suppressed matrix metalloproteinase 9 (MMP-9) and urokinase-type plasminogen activator (uPA) system. In SW620 (A) and SW480 (B) cells, the proinvasion factors MMP-9, uPA, and uPA receptor (uPAR) were tested by Western blotting. **p < 0.01 versus NC group.