Figure 3.

PFN2 overexpression promoted tumor cell proliferation and inhibited cell apoptosis. (A) Stable overexpression of PFN2 in OSC-19 cells was verified by Western blot. (B) Cell viability under PFN2 overexpression was examined by CCK-8 assay. (C, D) Colony formation of HNSC cells was significantly enhanced by PFN2 overexpression. (E, F) The proportion of apoptotic cells decreased after PFN2 overexpression, as assayed by annexin V/7-AAD staining. Data were expressed as mean ± SEM from five independent experiments, n = 5. *p < 0.05, **p < 0.01.