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. 2018 Dec 27;27(1):107–115. doi: 10.3727/096504018X15205622257163

Figure 4.

Figure 4

Rac1 was negatively regulated by miR-200a. GL15 cells were transfected with miR-200a mimic, miR-200a inhibitor, or their corresponding negative controls (mimic control and inhibitor control). The (A) mRNA and (B) protein level expressions of Rac1 were, respectively, detected by quantitative real-time PCR (qRT-PCR) and Western blot analyses. (C) A diagram showing the miR-200a that formed a base pair with the 3′-UTR of Rac1. Luciferase reporter activity in GL15 cells was detected after cotransfection with miR-200a mimic (mimic NC as control) and the vector containing the Rac1-wild type (Rac1-wt) or Rac1-mutated type (Rac1-mt). Data are presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. Data were expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA).