1,25(OH)2D3 attenuates IL-1β-induced increased proliferation and invasion in colorectal cancer (CRC) cells. Two CRC cell lines, DLD1 and LoVo, were used for the following experiments. (A) Proliferation of CRC cells assessed by CCK-8 assays. 1,25(OH)2D3 (100 nM) inhibits CRC cell proliferation that was promoted by 10 ng/ml of IL-1β. Error bar: mean ± SEM. *, #, ▴ p < 0.05. (B) Cancer cell invasion was measured by Transwell assay. (C) Cells that had moved through the upper chamber were counted. The Ctrl group number was normalized to 1. The values represent the means ± SEM. ***p < 0.001.