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. 2019 Jul 12;27(7):739–750. doi: 10.3727/096504018X15360541345000

Figure 3.

Figure 3

1,25(OH)2D3 attenuates IL-1β-induced EMT and increases the expression of vitamin D receptor (VDR). (A) Effect of 1,25(OH)2D3 and IL-1β on mRNA expression of E-cadherin, vimentin, and VDR in DLD1, and LoVo cells were detected by qRT-PCR. β-Actin was used as an internal control. (B, C) The expressions of E-cadherin, vimentin, and VDR in DLD1 and LoVo cells were detected by Western blot after treatment with 1,25(OH)2D3 and IL-1β. GAPDH was used as an internal control. Data represent the mean ± SEM. *p < 0.05, **p < 0.01.