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. 2019 Jul 12;27(7):739–750. doi: 10.3727/096504018X15360541345000

Figure 6.

Figure 6

lncTCF7 is transcriptionally regulated by VDR in response to 1,25(OH)2D3 stimulation. (A) A schematic representing the 10 predicted VDR binding sites in lncTCF7 promoter region, the reporter construct lncTCF7-Luc, and its truncated and mutated sites. (B) After treatment with 1,25(OH)2D3 for 24 h, transcription activity in DLD1 cells was measured by luciferase assay with a series of deletion mutants of lncTCF7-luc (internal control, pRL-TK). (C) After treatment with 1,25(OH)2D3 for 24 h, relative luciferase activity in DLD1 cells was detected by luciferase assay with VIE mutants of lncTCF7-luc. (D) DLD1 cells stimulated with 100 nM 1,25(OH)2D3 for 24 h was chromatin immunoprecipitated with the specific antibodies. Immunoprecipitated DNAs or chromatin was performed by PCR using a specific primer of the lncTCF7 promoter. Data represent the mean ± SEM. *p < 0.05.