Figure 4.

NOTCH3 correlated with COL1A1 as predictors for poor outcome of lung adenocarcinoma (LUAD) patients treated with TKIs. (A) Immunohistochemical staining of COL1A1 in LUAD and matched normal tissues. Scale bar: 50 μm. Positive staining is located in the cytoplasm. (B) COL1A1 mRNA expression was increased significantly in LUAD tissues compared with normal tissues (20 pairs). The level of COL1A1 mRNA expression in LUAD tissues was positively correlated with NOTCH3 in our cohort (n = 20; C) and TCGA data (n = 445; D). Patients with high expression of NOTCH3 protein (E) or COL1A1 protein (F) were associated with shorter overall survival.