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. 2019 Mar 29;27(4):431–437. doi: 10.3727/096504018X15201058168730

Figure 3.

Figure 3

FOXA1 was a direct target of miR-132 in thyroid cancer cells. (A) The predicted binding sites for miR-132 in the 3′-untranslated region (3′-UTR) of FOXA1 (positions 164–171) and the mutations in the binding sites are shown. (B) Luciferase activities were determined in TPC1 cells 48 h after cotransfection with reporter vectors carrying either wild-type FOXA1 3′-UTR (Wt-FOXA1) or mutated (Mut-FOXA1), and miR-132 mimic or miR-Ctrl. FOXA1 expression at the mRNA level (C) and protein level (D) was detected in TPC1 cells transfected with miR-132 mimic or miR-Ctrl. GAPDH was used as the internal control. **p < 0.01.