Figure 6.
Mediation of epithelial–mesenchymal transition (EMT) pathway activity by miR-148-3p or 5-Aza. (A) Western blot bands of N-cadherin, E-cadherin, vimentin, MMP-2, and MMP-9 in U87 cells and semiquantification of relative protein expression after miR-148-3p mimics transfection and/or 5-Aza treatment in U87 cells. (B) Western blot bands of N-cadherin, E-cadherin, vimentin, MMP-2, and MMP-9 in U251 cells, and semiquantification of relative protein expression after miR-148-3p mimics transfection and/or 5-Aza treatment in U251 cells. All data were presented as mean ± SD from three biological replicates. *p < 0.01 using one-way ANOVA followed by Tukey post hoc test.