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. 2019 Jun 21;27(6):653–661. doi: 10.3727/096504018X15420748671075

Figure 2.

Figure 2

Effect of AFAP1-AS1 on growth of ccRCC cells. (A) The expression level of AFAP1-AS1 in ccRCC cells was much higher than that in normal kidney epithelial HK-2 cells. (B, C) Knockdown of AFAP1-AS1 expression using specific shRNA. ShAF dramatically suppressed AFAP1-AS1 expression in Caki-1 and ACHN cells, which was determined by qRT-PCR compared with the control shRNA (shNC). (D, G) Caki-1 and ACHN cells transfected with shAF and shNC for 24, 48, and 72 h, cell growth was determined using CCK-8 assay. Effect of knockdown of AFAP1-AS1 on proliferating cell nuclear antigen (PCNA) expression was measured by Western blot analysis. Representative blots in Caki-1 (E, F) and ACHN (H, I) cells are shown. The data are shown as mean ± SD of three separate times. Significant difference between the shNC and shAF group was compared, *p < 0.05; **p < 0.01; ***p < 0.001.