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. 2019 Jun 21;27(6):653–661. doi: 10.3727/096504018X15420748671075

Figure 6.

Figure 6

In vivo biological role of AFAP1-AS1 on tumor growth of ccRCC. Nude mice were transplanted subcutaneously with Caki-1 cells transfected with shAF or the control shNC (n = 6). After 21 days, the mice were sacrificed. (A) Relative expression of AFAP1-AS1 in tumor tissue was determined by qRT-PCR. (B) A representative picture of the morphology of tumor xenografts after excision at 21 days of treatment. (C) Tumor weights were measured after the mice were sacrificed. (D) Tumor volume was also calculated by the formula in the Materials and Methods section. (E, F) The effect of knockdown of AFAP1-AS1 on the expression of EMT-related target proteins in xenograft tumors was analyzed by Western blot analysis. A representative immunoblot is shown. Values are presented as mean ± SD from three separate experiments. (G) Silencing of AFAP1-AS1 inhibits growth and metastasis of ccRCC via negative regulation of PTEN/AKT signaling. Significant difference between the shNC group and the shAF group was compared, *p < 0.05; **p < 0.01; *** p < 0.001.