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. 2018 Dec 27;27(1):99–106. doi: 10.3727/096504018X15195193936573

Figure 4.

Figure 4

miR-137 targets and inhibits enhancer of zeste homolog 2 (EZH2). (A) The TargetScan tool showed the binding sequence between miR-137 and EZH2 mRNA 3′-UTR. (B) Luciferase report assay showed the miR-137 mimic could obviously inhibit the luciferase activity of EZH2 3′-UTR-wt, but not EZH2 3′-UTR-mut. (C) The mRNA and protein expression levels of EZH2 in CRC tissues and adjacent normal colonic epithelial tissues. (D) The mRNA and protein expression levels of EZH2 in CRC cells with different metastatic potential. (E) LoVo cells were transfected with miR-137 mimic and/or pc-XIST. RT-qPCR showed the miR-137 expression in the different transfected cells. (F) The expression of EMT markers, including E-cadherin, N-cadherin, and vimentin, in the different transfected cells. Data were expressed as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to corresponding control.