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. 2019 Mar 29;27(4):449–458. doi: 10.3727/096504017X15016337254623

Figure 4.

Figure 4

ErbB3 was a direct target of miR-125a-5p. (A) Schematic representation of ErbB3 3′-untranslated regions (3′-UTRs) showing putative miRNA target site. (B) The mRNA expression of ErbB3 was determined by qRT-PCR in HepG2.2.15 and HepG3X cells transfected with miR-125a-5p mimic or miR-NC. (C) The protein expression of ErbB3 was determined by Western blot in HepG2.2.15 and HepG3X cells transfected with miR-125a-5p mimic or miR-NC. α-Tubulin was detected as a loading control. (D) The analysis of the relative luciferase activities of ErbB3-wild type (WT) or ErbB3-mutant (MUT) in HepG2.2.15 and HepG3X cells. All data are presented as mean ± SEM, n = 6. ##p < 0.01 versus miR-NC.