Figure 2.

H₂O₂ enhanced induction of metalloproteinase 2 (MMP2), MMP9, and intercellular adhesion molecule 1 (ICAM1) expression in response to TNF-α. HepG2 cells were stimulated with TNF-α (10 ng/ml) for 4 h either in the absence or in the presence of varying concentrations of H₂O₂. (A, B) MMP2 and MMP9 mRNA transcript levels were quantified by comparative real-time PCR and were normalized by measuring β-actin. (C) Expression of ICAM1 was compared by Western blot between HepG2 cells treated with TNF-α stimulation and varying concentrations of H₂O₂.