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Reversible Cezanne oxidation in vitro. Cezanne was first incubated with indicated concentrations of H2O2 for 15 min at 23°C, and 100 U of catalase was added to quench the H2O2. To one-half of the sample, DTT was added to a final concentration of 10 mM and incubated at room temperature for 15 min. The activity of the treated Cezanne was tested in a DUB assay using Lys48-linked diUb as substrate.
