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. 2019 Sep 23;27(9):1061–1068. doi: 10.3727/096504019X15565325878380

Figure 3.

Figure 3

miR-186 inhibits the migration, invasion, and epithelial–mesenchymal transition (EMT) of CCA cells. Transwell assay was performed to determine the migration (A) and invasion (B) ability of CCLP1 and SG-231 cells. Representative images showed migrating and invasive cells in the lower chamber stained with crystal violet. (C) Expressions of Twist1, E-cadherin, N-cadherin, vimentin, and metallopeptidase 9 (MMP9) were measured by Western blotting in CCLP1 cells. (D) Expressions of Twist1, E-cadherin, N-cadherin, vimentin, and MMP9 were measured by Western blotting in SG-231 cells. Data of (A) and (B) are presented as mean ± SD of at least three independent experiments *p < 0.001 versus control.