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. 2019 Feb 5;27(2):147–155. doi: 10.3727/096504017X15021536183517

Figure 1.

Figure 1

Inversed expression level of microRNA-26b (miR-26b) and cyclooxygenase-2 (COX-2) was observed in glioma tissues and cell lines. (A) The expression levels of miR-26b in glioma tissues and matched adjacent normal tissues by quantitative (q)RT-PCR. (B) The messenger RNA (mRNA) expression of COX-2 in glioma tissues and matched adjacent normal tissues was assessed by qRT-PCR assays. (C) The correlation between miR-26b expression levels and COX-2 mRNA expression levels in glioma tissues. (D) The expression levels of miR-26b in four glioma cell lines (U-87, C6, U-373, and M059J) and human astrocytes were measured by qRT-PCR. (E) The mRNA expression of COX-2 in glioma cell lines and astrocytes was assessed by qRT-PCR. (F) Western blotting assay was used to detect the expression profile of COX-2 in glioma cell lines and astrocytes. (G) Relative protein expression was quantified using Image-Pro Plus 6.0 software and normalized to GAPDH. Data are represented as the mean ± SD of three experiments. **p < 0.05 versus controls group.