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. 2019 Jun 21;27(6):729–737. doi: 10.3727/096504018X15443011011637

Figure 2.

Figure 2

Effects of DAC in combination with BZM on β-catenin and Wnt antagonists in myeloma cells. (A, B) Immunofluorescence images of β-catenin. Original magnification: 400×. (C) Protein lysates were subjected to immunoblotting with the indicated antibodies. GAPDH and lamin B1 were analyzed as controls. (D) Methylation-specific PCR (MSP) analysis of Wnt antagonists and DNMT3a in NCI-H929 cells. U indicates the presence of unmethylated genes, and M indicates the presence of methylated genes. *p < 0.05, **p < 0.01 versus the single treatment group.