Table 2.

Primer Sequences for Methylation-Specific PCR (MSP)

Primer for MSP	Forward Primer (5′→3′)	Reverse Primer (5′→3′)
DKK-1
Methylated	GTTTATTATTGAGAAGGGAGAATCG	ATAAACTTAATTAAACAAACGCGTA
Unmethylated	TTATTATTGAGAAGGGAGAATTGG	AATAAACTTAATTAAACAAACACATA
sFRP-3
Methylated	AAATATTTTTAGGAAGGAGGGTTTC	AAAATCGCAAACGATAAAATACG
Unmethylated	AAATATTTTTAGGAAGGAGGGTTTT	AAAATCACAAACAATAAAATACAAA
DNMT3a
Methylated	GAATTTTTCGAAGGAAAATTTTTTC	CTCCCTAACTAACACAAAACATAACG
Unmethylated	ATTTTTTGAAGGAAAATTTTTTTGT	CCCTAACTAACACAAAACATAACATA