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. 2019 Jul 12;27(7):789–799. doi: 10.3727/096504018X15444387696532

Figure 4.

Figure 4

Annexin A4 silencing also could inhibit the invasion and EMT in GC cells. SGC7901 cells were transfected with si-annexin A4 or si-NC. (A) The mRNA and protein levels of annexin A4 were determined by qRT-PCR and Western blot, respectively. (B) The invasion of SGC7901 cells was assessed by Transwell assay. (C) Levels of total MMP-2, MMP-9, and TIMP-1 were detected in the culture supernatants of cultured SGC7901 cells by ELISA assay. (D) The expressions of E-cadherin, N-cadherin, and vimentin were determined by qRT-PCR in SGC7901 cells. (E) The expressions of Twist1, Slug, and ZEB1 were determined by qRT-PCR in SGC7901 cells. All data are presented as mean ± SEM, n = 4. #p < 0.05, ##p < 0.01 versus si-NC.