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. 2019 Sep 23;27(9):1035–1042. doi: 10.3727/096504019X15528367532612

Figure 3.

Figure 3

miR-204 targeted at BIRC6 and increased p53 expression. (A) The human miR-204 with BIRC6 3′-untranslated region (3′-UTR) with seed sequence. (B) Luciferase activity of BIRC6 3′-UTR was examined in AML-5 or HL-60 cells treated with miR-204 mimics (miR-204-m, 20 nmol/L) or mimic negative control (NC-m) for 48 h. **p < 0.01 versus control, n = 6. (C) BIRC6 mRNA expression was determined by quantitative real-time PCR. **p < 0.01 versus control, n = 5. (D) Western blotting analysis of BIRC6. Representative images were shown. (E) Densitometric analysis of BIRC6 was performed. **p < 0.01 versus control, n = 6. (F) The cells were treated with miR-204 mimics for 48 h before ATO incubation (5 μmol/L). p53 expression was tested. (G) Densitometric analysis of BIRC6 was performed. **p < 0.01 versus control; ##p < 0.01 versus ATO, n = 5.