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. 2019 Feb 5;27(2):165–171. doi: 10.3727/096504018X15193506006164

Figure 4.

Figure 4

SOX9 was a target of miR-30c. (A) Diagram for the miR-30c binding sites in the 3′-UTR of SOX9 with the wild-type (WT) and mutated (Mut) sequences. (B) U87 and U251 cells were cotransfected with miR-30c and luciferase reporter constructs containing either pGL3-SOX9-3′-UTR-WT or pGL3-SOX9-3′-UTR-Mut. (C) The mRNA and (D) protein levels of SOX9 in U87 and U251 cells transfected with miR-30c mimics were measured by RT-qPCR and Western blot. (E) Pearson’s correlation analysis of the relative expression levels of miR-30c and the relative mRNA levels of SOX9. (F) RT-qPCR analysis for SOX9 expression in GBM tissues and adjacent normal tissues. All data are representative of three independent experiments and expressed as mean ± SD. **p < 0.01 and ***p < 0.001.