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. 2019 Feb 5;27(2):253–260. doi: 10.3727/096504018X15242763477504

Figure 4.

Figure 4

ZIC2 knockdown inhibited the proliferation, migration, and invasion of breast cancer cells. (A) Western blot was used to assess the protein levels of ZIC2 in MDA-MB-231 and BT549 cells transduced with siZIC2 or control. (B, C) CCK-8 and colony formation assays were used to measure the proliferation of MDA-MB-231 and BT549 cells transduced with siZIC2 or control. (D, E) The migration and invasion of MDA-MB-231 and BT549 cells were determined by Transwell assays. (F) The effect of ZIC2 on MDA-MB-231 and BT549 cell apoptosis was determined by annexin V/PI staining. *p < 0.05.