Figure 6.
Emodin inhibited tumor growth by suppressing EMT via inactivating the Wnt/β-catenin pathway in vivo. Animals were randomly divided into the control and emodin (40 mg/kg)-treated groups. After 7-day dosing of emodin by PO, 2 × 106 RKO cells were subcutaneously inoculated to the inguinal region of the mice to establish the CC xenograft model. (A) Tumor growth curves. (B) VEGF expression in tumor tissues by immunohistochemistry analysis. (C) Detection of E-cadherin and N-cadherin expression by Western blot. (D) Quantification of (C). (E) The effect of emodin on the expression of TCF4, cyclin D1, and c-Myc was valued by Western blot. (F) Quantification of (E). GAPDH was used as an internal reference. *p < 0.05 versus the control group.