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. 2019 Feb 5;27(2):211–218. doi: 10.3727/096504018X15208993118389

Figure 2.

Figure 2

TSP-1-overexpressing MG-63 cells inhibit human umbilical vein endothelial cell (HUVEC) tube formation and induce apoptosis. (A) HUVECs were seeded on Matrigel. Cultures were treated with either PBS, or culture medium (CM) from control MG-63 cells, or CM from MG-63 cells overexpressing TSP-1. HUVECs were incubated with both CM from MG-63 cells overexpressing TSP-1 and blocking CD36 antibody. Incubation of HUVECs with a blocking CD36 antibody abolished the inhibition of HUVEC tube formation induced by TSP-1 overexpression. Scale bar: 50 μm. The data are presented as means ± SD. **p < 0.01 compared to control, ##p < 0.01 compared to cells transfected with TSP-1. (B) Flow cytometric apoptosis assay. Cell apoptosis was assessed by flow cytometry. The data are presented as means ± SD. **p < 0.01 compared to control, ##p < 0.01 compared to cells transfected with TSP-1.