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. 2019 May 7;27(5):557–564. doi: 10.3727/096504018X15264647024196

Figure 2.

Figure 2

NLRP3 knockdown suppressed glioma cell growth and invasion through the decrease of interleukin-1β (IL-1β) and NF-κB p65. NLRP3 expression was transferred with shRNA in U87 and U251 cells to investigate the function of NLRP3 on cellular processes and the signal pathways involved. (A) The results of qRT-PCR to identify the silencing of NLRP3. (B) The results of Western blot to identify the silencing of NLRP3. (C) Cell counting kit-8 (CCK-8) assay for cell proliferation after NLRP3 silencing. (D) Transwell assay for cell invasion after NLRP3 silencing. All the pictures were obtained from the visual fields under a microscope (200×). (E) IL-1β secretion detected by enzyme-linked immunosorbent assay (ELISA). (F) Western blot results of IL-1β and NF-κB p65 expression. WT: normal cultured glioma cells; NC: glioma cells transfected with empty vectors; shNLRP3: glioma cells transfected with NLRP3-shRNA; ns: no significant difference. **p < 0.01. ***p < 0.001.