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. 2019 May 7;27(5):557–564. doi: 10.3727/096504018X15264647024196

Figure 4.

Figure 4

IL-1β elimination or NF-κB blockage abrogated NLRP3 hyperexpression and increased cell proliferation. U87 cells were transfected with NLRP3-pLVX and then treated with IL-1β neutralizing antibody or PDTC. (A) Forced expression of NLRP3 detected by qRT-PCR. (B) Forced expression of NLRP3 detected by Western blot. U87 cells were transfected with NLRP3-pLVX and/or treated with PDTC. (C) The expression of pro-IL-1β was measured by Western blot. (D) Cell growth detected by CCK-8 assay. WT: normal cultured U87 cells; NC: U87 cells transfected with empty vectors; pLVX-NLRP3: U87 cells transfected with NLRP3-pLVX; pLVX-NLRP3 + anti-IL-1β: U87 cells transfected with NLRP3-pLVX and treated with IL-1β neutralizing antibody; pLVX-NLRP3 + PDTC: U87 cells transfected with NLRP3-pLVX and treated with PDTC. ***p < 0.001.