Figure 3.

Knockdown of H19 attenuates high-mobility group AT-hook 2 (HMGA2)-mediated epithelial–mesenchymal transition (EMT). The mRNA (A) and protein levels (B) of HMGA2, vimentin, N-cadherin, and E-cadherin were confirmed by RT-qPCR and Western blot in H19-depleted CAL27 cells. GAPDH was probed as the loading control. The mRNA (C) and protein levels (D) of EMT-related transcription factors twist, zeb1, and snail1 were confirmed by RT-qPCR and Western blot. GAPDH was probed as loading control. Numbers are mean ± SD (n = 3, *p < 0.05 , **p < 0.01, and ***p < 0.001).