Table 2.
MicroRNA (miRNA) Mimic and miRNA Inhibitor Sequences, shRNA Sequence, and Primers
| Sequence 5′>3′ | Antisequence 5′>3′ | |
|---|---|---|
| mlet-7 | UUGUACUACACAAAAGUACUG | CUAUACAACCUACUACCUCAUU |
| ilet-7 | AACUAUACAACCUACUACCUCA | |
| shH19-1 | GAGTTAGCAAAGGTGACATCT | |
| shH19-2 | GACCTCATCAGCCCAACATCA | |
| shNC | TTCTCCGAACGTGTCACGTTTC | |
| β-actin | ATCAAGATCATTGCTCCTCCTGAG | CTGCTTGCTGATCCACATCTG |
| H19 | GCACCTTGGACATCTGGAGT | TTCTTTCCAGCCCTAGCTCA |
| HMGA2 | AAGTTGTTCAGAAGAAGCCTGCTCA | TGGAAAGACCATGGCAATACAGAAT |
| Vimentin | ATTCAAGCATTCCAGCTCTCA | TTGACTGACCCTTGAAGTGGCGAT |
| E-cadherin | CCTAGACTTGTGCCATGATGC | ACCTAGCAAGTCCCGTCAACCA |
| N-cadherin | TGCCAACTGAAGTCGTGGATT | GGCACACCTGGTACCACTTTACA |
| Twist1 | TGACCTAACGTGGTACCGTAA | CGGATCCGATTTCCCGCGTTT |
| Zeb1 | ACGGTACTTAGGCAACCTGAC | TTAGCCTAGCCCGTAATGGCTT |
| Snail1 | TTACGGATCGGTAAACCGTTA | TTACGATCGAAGTACGTAATGC |
mlet-7, let-7 mimic; ilet-7, let-7 inhibitor; shH19, short hairpin H19; shNC, short hairpin negative control; HMGA2, high-mobility group AT-hook 2.