Skip to main content
. 2019 Feb 5;27(2):173–182. doi: 10.3727/096504018X15202945197589

Table 2.

MicroRNA (miRNA) Mimic and miRNA Inhibitor Sequences, shRNA Sequence, and Primers

Sequence 5′>3′ Antisequence 5′>3′
mlet-7 UUGUACUACACAAAAGUACUG CUAUACAACCUACUACCUCAUU
ilet-7 AACUAUACAACCUACUACCUCA
shH19-1 GAGTTAGCAAAGGTGACATCT
shH19-2 GACCTCATCAGCCCAACATCA
shNC TTCTCCGAACGTGTCACGTTTC
β-actin ATCAAGATCATTGCTCCTCCTGAG CTGCTTGCTGATCCACATCTG
H19 GCACCTTGGACATCTGGAGT TTCTTTCCAGCCCTAGCTCA
HMGA2 AAGTTGTTCAGAAGAAGCCTGCTCA TGGAAAGACCATGGCAATACAGAAT
Vimentin ATTCAAGCATTCCAGCTCTCA TTGACTGACCCTTGAAGTGGCGAT
E-cadherin CCTAGACTTGTGCCATGATGC ACCTAGCAAGTCCCGTCAACCA
N-cadherin TGCCAACTGAAGTCGTGGATT GGCACACCTGGTACCACTTTACA
Twist1 TGACCTAACGTGGTACCGTAA CGGATCCGATTTCCCGCGTTT
Zeb1 ACGGTACTTAGGCAACCTGAC TTAGCCTAGCCCGTAATGGCTT
Snail1 TTACGGATCGGTAAACCGTTA TTACGATCGAAGTACGTAATGC

mlet-7, let-7 mimic; ilet-7, let-7 inhibitor; shH19, short hairpin H19; shNC, short hairpin negative control; HMGA2, high-mobility group AT-hook 2.