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. 2019 Feb 5;27(2):261–268. doi: 10.3727/096504018X15219188894056

Figure 3.

Figure 3

E2F8 was a target of miR-223-5p. (A) Diagram of predicted binding site of miR-223-5p in the 3′-UTR of E2F8 mRNA. (B) Luciferase reporter assay indicated that overexpression of miR-223-5p repressed the luciferase activity in H1299 cells transfected with wild-type (WT)-E2F8-3′-UTR. (C, D) qRT-PCR and Western blot analysis indicated that overexpression of miR-223-5p inhibited the mRNA and protein levels of E2F8 in H1299 cells. (E) Correlation of miR-223-5p expression with E2F8 level in NSCLC tissues. (F) Relative expression of E2F8 in NSCLC tissues and adjacent normal tissues. (G) Relative expression of E2F8 in NSCLC cell lines by qRT-PCR. *p < 0.05.