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. 2019 Feb 21;27(3):283–292. doi: 10.3727/096504017X15035795904677

Figure 4.

Figure 4

G15 inhibited GPER-mediated proliferation stimulated by E2 and G1 in H1793 cell lines. (A) The synchronized H1793 cells were treated with E2 or G1 at different concentrations (1, 10, and 100 nM), or fulvestrant (0.1, 1, and 10 μM) and G15 (0.1, 1, and 10 μM) in combination with 10 nM E2 and 10 nM G1 for 2 days. The viability of cells was analyzed using the Cell Counting Kit-8 assay. (B) The synchronized H1793 cells were treated with E2 (10 nM), G1 (10 nM), E2 + Ful (1 μM), E2 + G15 (1 μM), G1 + G15, and E2 + Ful + G15 for 1–5 days. The Cell Counting Kit-8 was used to evaluate the viability of cells. The OD value proportional to the cell number was measured and plotted on the growth curve. (C) The synchronized H1793 cells were treated with E2 (10 nM), G1 (10 nM), E2 + Ful (1 μM), E2 + G15 (1 μM), G1 + G15, and E2 + Ful + G15 for 2 days. The EdU assay of relative Hoechst-stained cells and EdU add-in cells was used to analyze cell proliferation. The data represent mean ± SEM from three different experiments (*p < 0.05 vs. control group, #p < 0.05 vs. E2 group).