Figure 5.

G15 inhibited GPER-mediated proliferation stimulated by E2 in urethane-induced adenocarcinoma mice. (A) Kunming mice were allowed to acclimatize to the environment for 1 week and then underwent an ovariectomy. One week later, animals were intraperitoneally administered with urethane. One week later, mice received 13 weeks of blank control, E2, G1, E2 + Ful, G1 + G15, E2 + Ful + G15, Ful, and G15 subcutaneously twice a week. (B) Gross examination of urethane-induced lung tumors in different groups and representative image of adenocarcinoma [hematoxylin and eosin (H&E) staining]. Original magnification: 100× (left) and 400× (right). (C, D) The body weight and number of nodules in the lungs (number of lung nodules in every mouse of each group) in different groups. (E) GPER protein expression in tumor tissues from different groups was detected using Western blot. The data represent means ± SEM (*p < 0.05 vs. control group, #p < 0.05 vs. E2 or G1 group, respectively).