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. 2019 Mar 29;27(4):459–467. doi: 10.3727/096504018X15193500663936

Figure 5.

Figure 5

IRS1 mediates the suppressive roles of miR-664 in MCF-7 and MDA-MB-231 cells. (A) Western blot analysis was applied to examine the IRS1 protein expression in MCF-7 and MDA-MB-231 cells cotransfected with miR-664 mimics and pcDNA3.1 plasmid or IRS1 overexpression plasmid pcDNA3.1-IRS1. *p < 0.05 compared with miR-NC, and miR-664 mimics + pcDNA3.1-IRS1. (B) CCK-8 and (C) Transwell invasion assays were conducted to determine the proliferation and invasion of MCF-7 and MDA-MB-231 cells after cotransfection with miR-664 mimic and pcDNA3.1 plasmid or pcDNA3.1-IRS1. *p < 0.05 compared with miR-NC, and miR-664 mimic + pcDNA3.1-IRS1.