Table 2.

Calculations of ΔΔG between wild-type and mutant Nucleocapsid Phosphoprotein. mCSM webserver was used to calculate the predicted ΔΔG. The negative values indicate the destabilization of protein upon mutation.

S. No.	PDB File	Wild-type Residue	Residue Position	Mutant Residue	Predicted ΔΔG (kcal.mol−1)	Outcome
1	6vyo.pdb	R	92	S	-1.146	Destabilizing
2	6vyo.pdb	G	120	R	-0.432	Destabilizing
3	6vyo.pdb	L	139	F	-0.94	Destabilizing
4	6vyo.pdb	A	152	S	-0.3	Destabilizing
5	6vyo.pdb	A	156	S	-1.422	Destabilizing
6	6wji.pdb	P	302	S	-1.475	Destabilizing
7	6wji.pdb	P	344	S	-0.346	Destabilizing
8	6wji.pdb	D	348	Y	-0.006	Destabilizing
9	6wji.pdb	T	362	I	-0.1	Destabilizing