Fig. 2.

Chd1 sliding activity is controlled by the location of the DNA-binding domain on entry or exit DNA. (A) Domain organization of Chd1[wt] and the chimeric Chd1[∆DBD/+AraC] fusion remodeler. Magenta represents the GSD helix, and green represents the bridge. (B) Sliding experiments using 50N0 streptavidin/biotin-H3X/H3Y nucleosomes, made with 601[swap SHL2.5/3.5], containing the araI1 sequence on extranucleosomal DNA. Sliding experiments were carried out with 150 nM 50N0 nucleosomes, 1 µM Chd1 variant, 10 µM streptavidin, and 2 mM ATP. (C) The Cy5 scan for experiments shown in B, which focuses on orientation B nucleosomes. Cartoon schematics highlight the positioning of the extranucleosomal DNA and araI1 sequence (orange) for different cross-linked products.