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. 2021 Jan 20;118(4):e2014498118. doi: 10.1073/pnas.2014498118

Fig. 5.

Fig. 5.

A model for allosteric regulation of the Chd1 ATPase motor on the nucleosome. The ATPase motor can sample active (Left) and autoinhibited states (Right) on the nucleosome. These states differ primarily by the location of ATPase lobe 2, which is fully engaged with nucleosomal DNA when active or held away from DNA in the inhibited form. The H4 tail (black) stabilizes the active form, whereas the chromodomains and GSD/bridge reinforce the inhibited form. The location of the DNA-binding domain allosterically regulates the GSD helix/bridge, favoring activation on entry DNA. By blocking full engagement with DNA, autoinhibitory elements cause the ATPase to bypass the twist defect cycle and therefore prevents the next ATP binding and hydrolysis event from stimulating DNA translocation.