Fig. 6.
OGT’s noncatalytic roles are required for cell proliferation. (A) Cell growth over time of all FKBP12F36V-OGT cell lines, as measured by live cell imaging. dTAG-13 treatment began at t = 0. Relative cell number is based on normalized measurements of confluence from live cell imaging. Error bars are SD (n = 6 per condition). (B) Growth outcomes for the Cre-based OGT deletion (Fig. 2) and dTAG-based OGT degradation systems (A). The degron-tagged ΔOGT cell line is viable because a very small amount of OGT remains after dTAG-13 treatment. (C) Schematic illustrating that OGT integrates catalytic and noncatalytic functions to promote cell growth.