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. 2021 Jan 19;118(4):e2019554118. doi: 10.1073/pnas.2019554118

Fig. 2.

Fig. 2.

Conjugation of PEG stabilizes LANA in living cells. (A) Structures of PEG-LANA-FITC (6–10). (B) Serum stability assay of 6–10. Each peptide was incubated with 25% human-serum-containing medium at 37 °C for indicated times. (C) Representative images of PEG-LANA-FITC 6–10 in living cells. HeLa cells expressing H2B-HaloTag were stained with HaloTag TMR Ligand and loaded with PEG-LANA-FITC. (Scale bar, 10 µm.) (D) Stability of 7–9 in living HeLa cells. Fluorescence images of PEG-LANA-FITC-loaded cells were acquired every 30 min and nuclear intensities were measured. Graphs represent relative fluorescence intensities (averages with SDs; n = 12 each). (E) Photobleaching was performed using HeLa cells loaded with 7–9. Average intensities with SDs (n = 20) are shown with curves after fitting (black lines). Half-time recoveries are also indicated.