Figure 6.

Human-specific GAPDH qRT-PCR correlates with and is more sensitive than histological quantification of lung metastasis by H&E staining of fixed thin sections

(A) Summary of the lung metastasis quantified in all of the experimental samples determined by human-specific GAPDH qRT-PCR (left, number of MDA-MB-231 cells/lung lobe) versus histological quantification by H&E staining of thin sections (right, percentage of MDA-MB-231 metastasis area averaged over 4 lobes of lung tissue). (B) The MDA-MB-231 lung metastasis values determined in every experimental sample by human-specific GAPDH qRT-PCR (x axis) versus histological quantification of H&E-stained, fixed thin sections (y axis) are plotted, and the Pearson correlation (R) is calculated.