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. 2021 Jan 22;118(4):e2011577118. doi: 10.1073/pnas.2011577118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 6. — PrpA is labile and degradation occurs at both termini. (A) The stability of N^FLAG-PrpA-C^His was determined by Tricine-SDS-PAGE (Upper), Western blot using anti-His antibody (Middle), and anti-FLAG antibody (Lower) after being treated with P. rubra cell lysates. Lane 1, size marker; lane 2, cell lysate; lane 3, purified N^FLAG-PrpA-C^His, lanes 4–9, N^FLAG-PrpA-C^His treated with P. rubra lysates over time. (B) Schematic of the modular organization of PrpA. (C) A BACTH assay was performed to assess interactions between PrpA proteins of varying lengths and PrpT.