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Extracellular vesicle separation by ultracentrifugation step-by-step method overview
(A) Media filter sterilization (0.22 μm filter) for subculturing cells.
(B) MCF10A cell pellet after centrifugation at 3,000 rpm for 10 min at 4°C.
(C) Two ultracentrifugation tubes balanced with media and using cold PBS as necessary. Ultracentrifuge tubes are filled to avoid collapse as a result of insufficient volume.
