Reagent | Stock concentration | Final concentration | Amount |
---|---|---|---|
DMEM high glucose medium | N/A | N/A | 500 mL |
FBS | N/A | 5% | 25 mL |
Insulin | 2.5 mg/mL | 1% | 2 mL |
Penicillin/streptomycin | 10,000 U/mL | 100 U/mL | 5 mL |
Reagent | Stock concentration | Final concentration | Amount |
---|---|---|---|
DMEM high glucose medium | N/A | N/A | 500 mL |
FBS | N/A | 5% | 25 mL |
Insulin | 2.5 mg/mL | 1% | 2 mL |
Penicillin/streptomycin | 10,000 U/mL | 100 U/mL | 5 mL |