Figure 1.

Miro2 is a direct target of miR-351-5p

(A) Sequence alignment of miR-351-5p with the 3′ UTR of Miro2. Luciferase assay constructs with either WT or MT 3′ UTR of Miro2 were prepared. (B) 293T cells were transfected with miR-351-5p, miR-Con, or anti-miR-351-5p with luciferase assay constructs. miR-351-5p inhibited luciferase expression by directly targeting the 3′ UTR of Miro2. (C) HCN cells were transfected with miR-351-5p or miR-Con. RT-qPCR analysis indicated that miR-351-5p downregulated Miro2 mRNA. (D and E) After transfection of HCN cells with miR-Con or miR-351-5p, western blot analysis showed downregulation of Miro2. miR-351-5p specifically downregulated Miro2 at both the mRNA and protein levels. The data represent the mean ± SD values (n > 3). ∗∗p < 0.01, ∗∗∗p < 0.001.