Figure 2.

Overexpression of miR-351-5p or suppression of its target candidate Miro2 induced cell death in HCN cells

(A and B) HCN cells were transfected with miR-Con, miR-351-5p, anti-miR-Con, or anti-miR-351-5p. After staining with Hoechst 33342 and PI at 24 h after transfection, cell death was quantified by counting PI-positive cells. Anti-miR-351-5p specifically inhibited miR-351-5p-induced cell death in a dose-dependent manner. (C and D) HCN cells were transfected with siCon or siMiro2. After 24 h, cell death was determined using the method described herein. Suppression of Miro2 significantly induced cell death in HCN cells. Data in the graphs represent the mean ± SD values (n > 3). ∗∗p < 0.01.