Figure 7.

Overexpression of Miro2 adequately recovered cell death and mitochondrial fission induced by miR-351-5p or insulin withdrawal on HCN cells

(A) Adenovirus expressing Miro2 was generated. Infection efficiency was confirmed using rAD-GFP virus used as a control (>90%, Figures S4A and S4B). rAD-Miro2 completely suppressed miR-351-5p-induced cell death. (B and C) Mitochondrial morphology was monitored and quantitated using MitoTracker after co-treatment with rAD-Miro2 and miR-351-5p. (D and E) HCN cells were cultured in an insulin (−) medium in the presence of rAD-GFP or rAD-Miro2 for 24 h. Cell death was measured by counting PI-positive cells. Mitochondrial morphology was monitored using MitoTracker under a confocal microscope. Data in the graphs represent the mean ± SD values (n > 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.