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. 2021 Jan 15;87(3):e01819-20. doi: 10.1128/AEM.01819-20

TABLE 1.

Sequences of degenerate primer pair and probe used in qPCR assays to detect the proposed methanogenic MIC biomarker micH

Primer or probea Sequence (5′→3′)b
micH-fwd AA(Y)CTTCTAACACCAA(M)TGA(Y)GGAAC
micH-rev TCAAATCCTCTAAATTCTGTGACACA
micH-probe TACCAACAGATAATGCTGCA
a

Proposed product length, 95 bp.

b

M = A or C; Y = C or T.