TABLE 1.
Kinetic parameters of Pm25
| Substrate | Activity (U/mg) | Km appb (mg/ml) | Kcat (s−1) | Kcat/Km (min−1 mM−1) |
|---|---|---|---|---|
| Beechwood GX | 7.4 ± 0.1 | 3.1 ± 0.2 | 10.3 ± 0.2 | 3.4 ± 0.1a |
| LVWAX | 25.8 ± 0.8 | 2.0 ± 0.2 | 36.2 ± 1.1 | 18.0 ± 0.5a |
| ADWAX | 21.7 ± 0.5 | 1.0 ± 0.1 | 30.4 ± 0.7 | 29.4 ± 0.7a |
| EDWAX | 21.4 ± 0.4 | 1.7 ± 0.1 | 29.9 ± 0.5 | 18.1 ± 0.3a |
| RAX | 30.3 ± 0.6 | 1.4 ± 0.1 | 42.4 ± 0.8 | 31.3 ± 0.6a |
| X6 | —c | — | — | 292.8 ± 36.8 |
| X5 | — | — | — | 35.5 ± 2.4 |
| X4 | — | — | — | 3.75 ± 0.1 |
| pNPX4 | — | — | — | 2381.7 ± 381.7 |
| pNPX3 | — | — | — | 1041.4 ± 138.2 |
| pNPX2 | — | — | — | 14.09 ± 0.66 |
a
For polysaccharide, kcat/Km app is in s−1 mg·ml−1.
b
Km app, apparent Km.
c
—, Not determined due to inability to saturate the enzyme under experimental conditions.