TABLE 2.
Binding of the recombinant proteins to soluble substrates as determined by affinity gel electrophoresis
| Ligand |
Kd fora: |
||||
|---|---|---|---|---|---|
| M1 | M7 | M8 | M9 | M10 | |
| RAX | 2.8 ± 0.1 | 18.8 ± 3.5 | 31.9 ± 0.8 | 18.1 ± 2.2 | 5.1 ± 0.4 |
| LVWAX | 3.7 ± 0.3 | 27.1 ± 1.9 | 22.7 ± 1.1 | 15.9 ± 1.9 | 3.7 ± 0.2 |
| ADWAX | 2.3 ± 0.2 | 15.6 ± 0.2 | 10.9 ± 0.7 | 8.0 ± 0.1 | 6.4 ± 0 |
| EDWAX | 2.0 ± 0.1 | 16.7 ± 0.4 | 12.6 ± 0.8 | 9.2 ± 0.4 | 3.2 ± 0.0 |
| Beechwood GX | 4.9 ± 0.3 | 62.0 ± 1.9 | 30.3 ± 1.2 | 17.5 ± 0.1 | 9.0 ± 0.5 |
| Nanocellulose | − | − | − | − | − |
| Galactomannan | − | − | − | − | − |
| Xyloglucan | + | + | + | − | + |
| Arabinan | − | − | − | − | − |
| β-Glucan | + | − | + | + | + |
a
The unit for Kd is mg·ml−1; +, retardation observable; −, no retardation.