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Persistence of defective virus (SfC) in mixtures with a homologous virus (SeA) during serial passage (P0 to P6) of mixed-virus OBs in Spodoptera exigua larvae. The presence of SfC was quantified by qPCR. The P0 initial inoculum was generated by injection of ODVs released from mixtures of SeA and SfC OBs (1:1 ratio) into S exigua larvae that subsequently died from virus disease. Error bars indicate SDs (n = 3).
